PNV35B

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• envase: tube of 5 μg 01080924-DNA-5UG; pkg of vial of cells 01080924-1VL
• ImmunoDon: BALB/c mice
• Mieloma: Ag876
• Reactividad: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
• técnicas: cell culture | mammalian: suitable
• isotipo: IgM plus IgG
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Anti-Osteoclastoma Hybridoma

Descripción línea celular

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

Inmunógeno

Osteoclastoma tumour

Aplicación

Immunocytochemistry

Medio de cultivo

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

Rutina de subcultivo

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

Otras notas

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