C9658
Carboxypeptidase G from Pseudomonas sp.
lyophilized powder, ≥3 units/mg protein
Sinónimos:
γ-Glutamyl hydrolase
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About This Item
Productos recomendados
formulario
lyophilized powder
Nivel de calidad
actividad específica
≥3 units/mg protein
mol peso
homodimer ~90 kDa
composición
Protein, ~70% biuret
solubilidad
H2O: soluble 0.5 mg/mL
temp. de almacenamiento
−20°C
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Aplicación
Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.
Acciones bioquímicas o fisiológicas
Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
Definición de unidad
One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
Forma física
Contains sodium acetate salt.
Nota de preparación
Chromatographically purified
Solutions should be prepared fresh prior to use.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Proceedings of the National Academy of Sciences of the United States of America, 71(10), 3906-3910 (1974-10-01)
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