C7512
Butyrylcholinesterase from equine serum
lyophilized powder, ≥10 units/mg protein
Sinónimos:
Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
formulario
lyophilized powder
actividad específica
≥10 units/mg protein
mol peso
tetramer 440 kDa
composición
Protein, ≥60%
solubilidad
cold water: 60 U/mL
temp. de almacenamiento
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter.
Aplicación
Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.
Acciones bioquímicas o fisiológicas
Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates.
Mutations in butyrylcholinesterase (BChE) gene results in the failure of respiratory system, prolonged paralysis and apnea.
Definición de unidad
One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.
Forma física
Lyophilized powder containing buffer salts
Nota de preparación
This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.
Nota de análisis
Protein determined by biuret
inhibidor
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Determinants of intracellular pH in the erythrocyte.
J Warth et al.
British journal of haematology, 29(3), 369-372 (1975-03-01)
Mauricio Piñeiro et al.
Microorganisms, 11(1) (2023-01-22)
Hippeastrum species have a wide range of biological properties. In Argentina, this genus comprises ten widely distributed species. To evaluate the antiparasitic and anticholinesterase activities and chemical profiles of seven Argentinean Hippeastrum species and determine the synergism between the major
Kohji Mitsubayashi et al.
Analytica chimica acta, 573-574, 69-74 (2007-08-29)
A novel bioelectronic sniffer for nicotine in the gas phase was developed with enzyme inhibition principle to butyrylcholinesterase activity. The bioelectronic devices for nicotine in the gas and liquid phases were constructed using a Clark-type dissolved oxygen electrode and a
Screening for cholinesterase inhibitors in selected fruits and vegetables.
Szwajgier D and Borowiec K
Biotechnology, 15(2) (2012)
Toxicokinetics of Chemical Warfare Agents: Nerve Agents and Vesicants
Handbook of Toxicology of Chemical Warfare Agents, 755-790 (2009)
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico