C-12352

Normal Human Dermal Fibroblasts (NHDF)

Adult, 500,000 proliferating cells

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: human dermis
• envase: pkg of 500,000 cells
• morfología: (fibroblast)
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: ambient
• temp. de almacenamiento: room temp

Descripción

Descripción general

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Origen línea celular

Skin

Aplicación

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of adult skin (different locations) and are provided in a proliferating format. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Calidad

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutina de subcultivo

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Otras notas

Recommended Plating Density: 3500 - 7000 cells per cm²Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Productos recomendados

Recommended Primary Cell Culture Media:Link

Recommended Primary Cell Culture Media:Link

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable