C0817

Monoclonal Anti-HLA-DQ antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Monoclonal Anti-HLA-DQ
• Código UNSPSC: 12352203

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: HK19, monoclonal
• Formulario: buffered aqueous solution
• reactividad de especies: human
• técnicas: flow cytometry: 5 μL using 1 × 10⁶ cells
• isotipo: IgG2b
• Nº de acceso UniProt: P01920
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HLA-DQB1(3119)

Descripción

Especificidad

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Inmunógeno

GM-1500 human tumor cell line.

Aplicación

Monoclonal Anti-HLA-DQ antibody produced in mouse is suitable for flow cytometry at a concentration of 5 μL using 1 × 10⁶ cells.

Acciones bioquímicas o fisiológicas

Human leukocyte antigen (HLA)-DQ is a cell surface receptor type protein found on antigen presenting cells. DQ is a αβ heterodimer of the MHC Class II type. It plays a central role in the immune system and is expressed in antigen presenting cells. HLA-DQ expression in cystic fibrosis CF is associated with inflammation and reduces response to Interferon γ (IFNγ). HLA-DQ acts as alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA) and antibody-mediated-rejection but does not support organ transplantation.

Descripción de destino

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide