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BCK-HTS488-2

Sigma-Aldrich

EdU HTS Kit 488

sufficient for 200 assays

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.22

fluorescencia

λex 496 nm; λem 516 nm

Condiciones de envío

wet ice

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Descripción general

The Baseclick EdU HTS Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis of adherent cells in 96 well plates. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU HTS Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU HTS Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Aplicación

High Throughput Screening

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C

Riesgos supl.

EUH032

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Jun Zou et al.
American journal of physiology. Gastrointestinal and liver physiology, 318(1), G10-G22 (2019-08-23)
Gastric cancer (GC) is one of the most common cancers in the world and remains a heavy burden of health worldwide. Adenylate cyclase 3 (ADCY3) is a widely expressed membrane-associated protein in human tissues and has been identified to be
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

Contenido relacionado

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.

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