This product has not been tested on microalgae.
APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Sinónimos:
Red fluorescent Annexin V apoptosis assay
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 914,00 € |
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352207
914,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleusage
sufficient for 200 tests
Quality Segment
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Legal Information
Cy3 is a trademark of Cytiva
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) protein quantification: suitable | technique(s) - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| usage sufficient for 200 tests | usage (20 tests) | usage 1 kit sufficient for 2,000 tests | usage 1 kit sufficient for 1,000 tests |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Solo componentes del kit
Referencia del producto
Descripción
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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can we use this kit used for microalgae cells
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