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Merck

A8792

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

HRP Rabbit Anti-Human IgG (whole molecule)

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162456
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
29

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biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:40,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Este artículo
A8917A8667A8419
Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8792

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8917

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8667

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat

Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8419

Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

goat

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

species reactivity

human

species reactivity

bovine

species reactivity

human

species reactivity

-

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, dot blot: 1:80,000 (chemiluminescent)

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

direct ELISA: 1:40,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Immunogen

purified human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)[1]
western blotting
microarray analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000480952
0000480952
0000459337
0000459337
0000447042

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Jenny Calow et al.
mAbs, 8(8), 1498-1511 (2016-10-30)
Antibody glycosylation is a key parameter in the optimization of antibody therapeutics. Here, we describe the production of the anti-cancer monoclonal antibody rituximab in the unicellular ciliate, Tetrahymena thermophila. The resulting antibody demonstrated enhanced antibody-dependent cell-mediated cytotoxicity, which we attribute
Rajika Lasanthi Dewasurendra et al.
BMC infectious diseases, 17(1), 49-49 (2017-01-11)
Sri Lanka achieved the WHO certificate as a malaria free country in September 2016, thus monitoring of malaria transmission using sensitive and effective tools is an important need. Use of age-specific antibody prevalence as a serological tool to predict transmission
Arnold Duralliu et al.
Pharmaceutics, 12(4) (2020-04-02)
High protein concentration products for targeted therapeutic use are often freeze-dried to enhance stability. The long-term storage stability of freeze-dried (FD) plasma-derived Immunoglobulin G (IgG) from moderate to high concentrations (10-200 mg/mL) was assessed. Monomer content, binding activity and reconstitution
Mônica Franca et al.
Journal of applied oral science : revista FOB, 15(3), 213-219 (2007-06-01)
Periodontitis is a chronic disease that results from an interaction of a mixed bacterial challenge and the host response. The purposes of this study were to evaluate the IgG serum levels to Porphyromonas gingivalis antigens by ELISA in individuals with
Xiaomin He et al.
Molecular medicine reports, 14(5), 4255-4262 (2016-10-26)
Chronic hepatitis B (CHB) is prevalent worldwide and can develop into liver cirrhosis and liver carcinoma. Early discrimination of liver cirrhosis from chronic hepatitis is critical for effective treatment and optimal prognosis. The aim of the present study was to
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Data Sheet - A8792

Instructions

Número de artículo de comercio global

SKUGTIN
A8792-2ML04061838443649

Preguntas

  1. what is the recommended dilution for western blotting

    1 respuesta

    1. The Western Blot picture is from the article in link below. However, It does not mention how the product is diluted:
      https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026032&type=printable

      Below are links to articles that have cited this antibody in Western Blot. Various dilutions are used. The end-user may titrate the product to find the best dilution for their application. The end-user may try something like 1:1000, 1:5000, 1:10,000, 1:20,000, etc.:

      https://www.researchgate.net/profile/Yuriy-Kit/publication/303736655_Identification_of_the_Unique_Properties_of_IgGs_and_their_Heavy_chains_in_Blood_serum_of_Multiple_Sclerosis_Patients/links/574ff60608aef199238f01e4/Identification-of-the-Unique-Properties-of-IgGs-and-their-Heavy-chains-in-Blood-Serum-of-Multiple-Sclerosis-Patients.pdf

      https://www.scielo.br/j/jaos/a/pBt9nVwJydK6Qz3qwMrNLrc/?lang=en

      https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.612781/full

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