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Merck

A7646

Apirasa from potatoes

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sinónimos:

Adenosina 5′-difosfatasa, Adenosina 5′-trifosfatasa

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Número CAS:
9000-95-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
MFCD00130542
Número CE:
3.6.1.5 (BRENDA, IUBMB)
Biological source:
potato

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biological source

potato

type

Grade III

form

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

mol wt

~49 kDa by gel filtration

composition

Protein, 30-60%

solubility

H2O: soluble 10 mg/mL, slightly hazy

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤5% of base activity

storage temp.

−20°C

Quality Level

200

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Este artículo
A6535SRE0019A6410
Apyrase from potatoes ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sigma-Aldrich

A7646

Apyrase from potatoes

Apyrase from potatoes ATPase ≥200 units/mg protein, lyophilized powder

Sigma-Aldrich

A6535

Apyrase from potatoes

Sigma-Aldrich

Sigma-Aldrich

SRE0019

Apyrase from potatoes

Apyrase from potatoes ATPase ≥200 units/mg protein, lyophilized powder

Sigma-Aldrich

A6410

Apyrase from potatoes

biological source

potato

biological source

-

biological source

-

biological source

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

form

lyophilized powder (partially purified)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 10 mg/mL, slightly hazy

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 1.0 mg/mL

solubility

H2O: soluble 1.0 mg/mL

mol wt

~49 kDa by gel filtration

mol wt

-

mol wt

-

mol wt

~49 kDa by gel filtration

Application

Se encuentran al menos dos isoenzimas en diferentes variedades de S. tuberosum: una con una elevada relación ATPasa/ADPasa (∼10) y otra con una relación baja (∼1).
Reacción: ATP → ADP+Pi → AMP+2Pi.
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.[1] The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.[2]

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis.[3] Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Packaging

Sold on the basis of ATPase units

Analysis Note

Enthält Säurephosphatase.

Other Notes

Mixture of high and low ratio isoenzymes.
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000511063
0000511063
0000500579
0000500575
0000500574

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Yen-Ta Chen et al.
Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
Marko Gosak et al.
Life (Basel, Switzerland), 11(5) (2021-05-01)
Ca2+ homeostasis and signaling disturbances are associated with lens pathophysiology and are involved in cataract formation. Here, we explored the spatiotemporal changes in Ca2+ signaling in lens epithelial cells (LECs) upon local mechanical stimulation, to better understand the LECs' intercellular
H J Kruse et al.
Arteriosclerosis and thrombosis : a journal of vascular biology, 14(11), 1821-1828 (1994-11-01)
The blocker of receptor-mediated calcium entry SK&F 96365 was used to evaluate the contribution of calcium influx to the formation of biologically active endothelial prostanoids and endothelium-derived relaxing factor (EDRF). SK&F 96365 inhibited histamine-stimulated calcium entry into human umbilical vein
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
The NMDA receptor regulates integrin activation, ATP release and arterial thrombosis through store-operated Ca2+ entry in platelets.
Reusswig, et al.
Frontiers in cardiovascular medicine, 10, 1171831-1171831 (2023)
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