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A5854

Sigma-Aldrich

Anti-AFX (FOXO4) antibody, Mouse monoclonal

clone AF3.10, purified from hybridoma cell culture

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About This Item

Número MDL:
MFCD03791410
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

AF3.10, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~50 kDa

reactividad de especies

human

técnicas

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using COS-7 cell extracts expressing human AFX

isotipo

IgG2b

Nº de acceso UniProt

P98177

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... FOXO4(4303)

Descripción general

Forkhead box O4 (FOXO4) belongs to the FOXO transcription factor family that regulates growth, cell cycle, metabolism, stress tolerance, aging and apoptosis in cells. Studies have reported that USP7 modulates FOXO4 transcription. Moreover, it has been demonstrated that peroxide stress acetylates FOXO4, whereas the longevity protein hSir2SIRT1 deacetylates it.

Especificidad

Monoclonal Anti-AFX (FOXO4) recognizes human AFX.

Aplicación

Monoclonal Anti-AFX (FOXO4) antibody can be used for western blot assays at 0.5-1μg/ml. The antibody is also suitable for use in indirect ELISA, immunocytochemistry and microarray applications.

Acciones bioquímicas o fisiológicas

Anti-AFX (FOXO4) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma AF3.10 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. AFX plays a central role in the regulation of cell proliferation. It integrates signals from PI3K/PKB and Ras/Ral signaling pathways to regulate transcription of the cell cycle inhibitor p27kip1. AFX activation results in increased expression of p27kip1, resulting in cell cycle arrest. Overexpression of AFX blocks cells cycle progression at G1 by a p27kip1 dependent mechanism.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27 kip1
Medema RH, et al.
Nature, 404(6779), 782-782 (2000)
Armando van der Horst et al.
The Journal of biological chemistry, 279(28), 28873-28879 (2004-05-06)
FOXO transcription factors have important roles in metabolism, cellular proliferation, stress tolerance, and aging. FOXOs are negatively regulated by protein kinase B/c-Akt-mediated phosphorylation. Here we show that FOXO factors are also subject to regulation by reversible acetylation. We provide evidence
Armando van der Horst et al.
Nature cell biology, 8(10), 1064-1073 (2006-09-12)
FOXO (Forkhead box O) transcription factors are important regulators of cellular metabolism, cell-cycle progression and cell death. FOXO activity is regulated by multiple post-translational modifications, including phosphorylation, acetylation and polyubiquitination. Here, we show that FOXO becomes monoubiquitinated in response to

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