A4140
Aequorin from jellyfish (Aequorea sp.)
Type III, solid
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About This Item
origen biológico
Aequorea sp.
tpo
Type III
Formulario
solid
uso
mg solid sufficient for ~200 calcium assays
composición
Protein, ~0.5% Bradford
aplicaciones
detection
temp. de almacenamiento
−20°C
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Descripción general
Package sizes are based on total solids content
Apoaequorin has a mol. wt. approx. 22 kDa, and contains a molecule of coelenterazine hydroperoxide as the chromophoric prosthetic group. Upon binding Ca2+, aequorin decomposes to apoaequorin, coelenteramide, and CO2, and emits light.
Acciones bioquímicas o fisiológicas
A bioluminescent protein; can be used to measure physiological levels of calcium in serum and subcellular organelles down to 10 μM.
Forma física
Contains buffer salts consisting mainly of sodium EDTA, pH 7.5
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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Satoshi Inouye et al.
Protein expression and purification, 83(2), 205-210 (2012-04-28)
Highly purified histidine-tagged aequorin with a reactive cysteine residue (His-Cys4-aequorin) was obtained from the periplasmic space of Escherichia coli cells by nickel-chelate affinity chromatography and hydrophobic chromatography. The procedure yielded 40.3mg of His-Cys4-aequorin from 2L of cultured cells with over
Denis Ottolini et al.
Methods in molecular biology (Clifton, N.J.), 937, 273-291 (2012-09-26)
In the last two decades the study of Ca(2+) homeostasis in living cells has been enhanced by the explosive development of genetically encoded Ca(2+)-indicators. The cloning of the Ca(2+)-sensitive photoprotein aequorin and of the green fluorescent protein (GFP) from the
Fen-Fen Lin et al.
The Journal of pharmacology and experimental therapeutics, 345(2), 225-238 (2013-03-12)
Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. There are two key components of this
Nazia Haq et al.
Assay and drug development technologies, 11(2), 93-100 (2012-10-11)
Gap junctions (GJs) are intercellular channels which are composed of the connexin family of proteins that allow electrical and chemical communications and synchronization in tissue ensembles. Evidence suggests that pharmaceutical modulators of these channels may have therapeutic potential or carry
Daiana Minocci et al.
Biochimica et biophysica acta, 1833(7), 1632-1640 (2013-01-05)
Different optical imaging techniques have been developed to study neuronal activity with the goal of deciphering the neural code underlying neurophysiological functions. Because of several constraints inherent in these techniques as well as difficulties interpreting the results, the majority of
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