A0604

Monoclonal Anti-Angiopoietin-1 antibody produced in mouse

clone 171718, purified immunoglobulin

• Sinónimos: Anti-ANG-1
• Número MDL: MFCD06798694
• Código UNSPSC: 51111800
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 171718, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: 25 μg/mL using cells and tissue sections; indirect ELISA: 0.5-1.0 μg/mL; western blot: 1-2 μg/mL
• isotipo: IgG2b
• Nº de acceso UniProt: Q15389
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ANGPT1(284)

Descripción

Descripción general

Anti-Angiopoietin-1 detects recombinant human angiopoietin-1. The antibody shows no cross-reactivity with recombinant human angiopoietin-2, recombinant human angiopoietin-4, recombinant mouse angiopoietin-like 3, and recombinant human cornea-derived transcript 6 (CDT-6).The ANG-1 gene is mapped to human chromosome 8q23.1.

Inmunógeno

purified, NSO-derived, recombinant human ANG-1.

Aplicación

Anti-Angiopoietin-1 antibody may be used for immunoblotting at a working concentration of 1-2 μg/mL. It has also been used for immunoblotting using skeletal muscle, kidney, lung, epididymal fat and brain tissue of mouse. For ELISA, the recommended concentration is 0.5-1.0 μg/mL. The antibody is suitable for immunohistochemistry at a working antibody concentration of 25 μg/mL.Monoclonal Anti-Angiopoietin-1 antibody produced in mouse has been used in Western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Acciones bioquímicas o fisiológicas

Angiopoietin 1 is an endothelial cell-specific protein that is a ligand for the TIE2 tyrosine receptor. It is required for mature blood vessel formation .
Angiopoietins are growth factors vital for vascular differentiation during normal development. There are four angiopoietins discovered that bind to receptor tyrosine kinases Tie2. Angiopoietin-1 (ANG-1) is a potent pro-angiogenic factor that works by recruits mural cells for endothelial tubule formation and vessel maturation. ANG-1 acts along with VEGF in enhance angiogenesis and increase vascular branching. ANG-1 also affects the inflammatory process and inhibits the capillary transmigration of leukocytes on TNF-α stimulation. Sonic hedgehog, a secreted growth factor, reportedly upregulates the expression of ANG-1. Overexpression of angiopoietins has been observed in a variety of malignancies including neuroblastoma, glioblastoma and lung cancers. While ANG-2 increases the metastasis and angiogenesis of the tumors, ANG-1 inhibits tumor angiogenesis

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)