B5525

Bromophenol Blue sodium salt

• Sinónimos: 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Fórmula empírica (notación de Hill): C₁₉H₉Br₄NaO₅S
• Número de CAS: 34725-61-6
• Peso molecular: 691.94
• Beilstein: 6995701
• Número CE: 252-170-2
• Número MDL: MFCD00013793
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 57653941
• NACRES: NA.52

Propiedades

• Formulario: powder
• Nivel de calidad: 200
• técnicas: electrophoresis: suitable
• solubilidad: water: soluble
• idoneidad: suitable for nucleic acid electrophoresis
• temp. de almacenamiento: room temp
• cadena SMILES: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• Clave InChI: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Descripción

Descripción general

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicación

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

Reconstitución

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)