99090226
HH-8 cell line
human kidney (embryonic)
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About This Item
Código UNSPSC:
41106514
Productos recomendados
product name
HH-8 cell line,
origen biológico
human kidney (embryonic)
formulario
liquid
modo de crecimiento
Semi-adherent aggregates
cariotipo
Not specified
morfología
Not specified
productos
Not specified
receptores
Not specified
técnicas
cell culture | mammalian: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Categorías relacionadas
Origen línea celular
Human embryo kidney
Descripción línea celular
The cell line HH-8 was generated by transfecting a vector (pcDNA3) containing a truncated calcium channel cDNA into (HEK) 293 cells (ECACC catalogue no. 85120602). The vector is constituitively expressed in the HH-8 cells producing a truncated (at amino acid 1733) cardiac α 1c-a calcium channel.
Medio de cultivo
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS + 200 μg/ml Geneticin
Rutina de subcultivo
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin, trypsin/EDTA, or PBS wash, 5% CO2; 37°C. Cells may take up to 7 days to attach after resuscitation and sub culture. Cells grow in large aggregates that do not grow to confluency. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and re-seed to allow re-attachment of cells.
Otras notas
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Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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S Dai et al.
FEBS letters, 442(1), 70-74 (1999-01-29)
Facilitation of calcium current by depolarizing prepulses has been observed in many cells including cardiac muscle. The mechanism underlying prepulse facilitation is controversial with respect to the requirements of channel subunits and cAMP kinase. We found that coexpression of the
C Seisenberger et al.
Naunyn-Schmiedeberg's archives of pharmacology, 352(6), 662-669 (1995-12-01)
Stable cell lines are potentially excellent tools for large-scale screening of new compounds. Two carboxyterminal-deleted constructs of the two splice variants a and b of the calcium channel class C alpha 1 subunit were expressed stably in HEK 293 cells.
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