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85639

SSC Buffer

Name: SSC Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Sinónimos:

Saline sodium citrate buffer dry blend

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A ustedes/SKU	Disponibilidad	Precio

Acerca de este artículo

NACRES:

NA.25

UNSPSC Code:

12161700

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Propiedades

grade

Molecular Biology

Quality Segment

100

product line

BioReagent

form

powder blend

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

solubility

H₂O: 0.263 g/mL, clear, colorless

storage temp.

room temp

Descripción

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Preparation Note

Convenient pack sizes
Multi-purpose use in hybridizations
Easy-to-use powder makes fast, inexpensive solutions

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Other Notes

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

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Este artículo	S0902	11666681001	S8140
Sigma-Aldrich 85639 SSC Buffer	Sigma-Aldrich S0902 SSC Buffer	Roche 11666681001 Buffers in a Box, Premixed SSC Buffer, 20x	Sigma-Aldrich S8140 SSPE Buffer
grade Molecular Biology	grade -	grade -	grade Molecular Biology
Quality Level 100	Quality Level 200	Quality Level -	Quality Level 200
product line BioReagent	product line -	product line -	product line -
form powder blend	form powder blend	form solution	form powder blend
solubility H₂O: 0.263 g/mL, clear, colorless	solubility water: 263 mg/mL, clear, colorless	solubility -	solubility water, high purity: 20 ×, clear, colorless
storage temp. room temp	storage temp. room temp	storage temp. 20-25°C	storage temp. room temp

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lot/Batch Number

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