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78102

Sigma-Aldrich

Anti-Mouse IgG - Atto 633 antibody produced in goat

~1 mg/mL IgG, affinity isolated antibody

Sinónimos:

Anti-Mouse IgG−Atto 633 antibody produced in goat, Atto 633 [goat-Anti-mouse IgG]

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

conjugado

Atto 633 conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

contiene

50% glycerol as stabilizer

reactividad de especies

mouse

concentración

~1 mg/mL IgG

fluorescencia

λex 636 nm; λem 650 nm in PBS

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Categorías relacionadas

Descripción general

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.

Inmunógeno

purified mouse IgG (whole molecule).

Aplicación

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spect

Forma física

Solution in phosphate buffered saline and 5 mM sodium azide, pH 7.5, with glycerol 1:1

Nota de análisis

Unconjugated dye ≤5% of total fluorescence

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Frases de peligro

H412

Consejos de prudencia

P273 - P501

Clasificaciones de peligro

Aquatic Chronic 3

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Charlotte Calvet et al.
iScience, 25(12), 105628-105628 (2022-12-10)
Hearing depends on fast and sustained calcium-dependent synaptic vesicle fusion at the ribbon synapses of cochlear inner hair cells (IHCs). The implication of the canonical neuronal SNARE complex in this exocytotic process has so far remained controversial. We investigated the
Tharun Selvam Mahendran et al.
Frontiers in aging neuroscience, 12, 537712-537712 (2020-12-01)
Aggregated tau is a hallmark neuropathological feature in numerous neurodegenerative disorders. Previous studies aiming to validate aggregated tau pathology as a pathogenic driver of neurodegeneration in correlation to characteristic behavioral phenotypes have had shortcomings. Although studies on soluble tau pathology
Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)

Artículos

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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