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Merck

70540

Naphthol Yellow S

suitable for microscopy

Sinónimos:

2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

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Fórmula empírica (notación de Hill):
C10H4N2Na2O8S
Número CAS:
846-70-8
Peso molecular:
358.19
EC Number:
212-690-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329762555
MDL number:
MFCD00003958
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220

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form

powder

Quality Level

200

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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Este artículo
49547A553322680
Naphthol Yellow S suitable for microscopy

Sigma-Aldrich

70540

Naphthol Yellow S

Naphthol Yellow S analytical standard

Supelco

49547

Naphthol Yellow S

Alizarin Red S certified by the BSC

Sigma-Aldrich

A5533

Alizarin Red S

Quinoline Yellow suitable for microscopy, mixture of mono- and disulfonic acid sodium salt

Sigma-Aldrich

22680

Quinoline Yellow

grade

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

grade

analytical standard

grade

certified by the Biological Stain Commission

grade

for microscopy (Hist.)

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

solubility

-

solubility

H2O: 1 mg/mL

solubility

H2O: 0.1 g/10 mL

form

powder

form

-

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.[1]

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H373

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
WE276745/1
276745/1
0000026057
0000027025

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
J Gaub
The Histochemical journal, 13(5), 717-722 (1981-09-01)
Owing to the accumulation of nuclear non-histone protein (NHP) (a) in cells entering the cell cycle from the quiescent state and (b) in continuously cycling cells during G1 phase, a simultaneous determination of DNA and nuclear NHP is of high
S Gutschmidt et al.
Histochemistry, 72(3), 467-479 (1981-01-01)
In order to quantify the amount of protein in the small intestinal brush border region at different villus sites, cryostat sections of adult rat jejunum were stained with Naphthol Yellow S, Dinitrofluorobenzene and Coomassie Brilliant Blue and the dye deposits
F A Bignone et al.
Bollettino della Societa italiana di biologia sperimentale, 57(9), 950-955 (1981-05-15)
In the present work we studied the reversibility of the binding reaction of Naphthol Yellow S to proteins using a Testing Automatic System Leitz. We presently think that the NY-S staining procedure, can be used as a reliable quantitative method
Cytospectral quantification for cellular dehydrogenases.
M O Yamada et al.
Cellular and molecular biology, including cyto-enzymology, 26(4), 347-352 (1980-01-01)
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