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69125

Resorufin methyl ether

Name: Resorufin methyl ether
Brand: SIGMA

suitable for fluorescence, ≥98.0% (HPLC)

Sinónimos:

7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O⁷-Methylresorufin

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About This Item

Fórmula empírica (notación de Hill):

C₁₃H₉NO₃

Número de CAS:

5725-89-3

Peso molecular:

227.22

Beilstein:

209529

Número MDL:

MFCD00037663

Código UNSPSC:

12352200

ID de la sustancia en PubChem:

57652175

Ensayo

≥98.0% (HPLC)

Formulario

crystals

mp

≥220 °C (lit.)

solubilidad

DMF: soluble
DMSO: soluble
alcohols: soluble

fluorescencia

λ_ex 571 nm; λ_em 585 nm in dealkylase(lit.)

idoneidad

suitable for fluorescence

temp. de almacenamiento

2-8°C

cadena SMILES

COc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3

Clave InChI

KNYYMGDYROYBRE-UHFFFAOYSA-N

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Aplicación

Used to differentiate isozymes of cytochrome P-450

Envase

Bottomless glass bottle. Contents are inside inserted fused cone.

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Sigma-Aldrich

90101

Ampliflu^™ Red

Sigma-Aldrich

R7017

Resazurin sodium salt

Effect of mutations at Lys250, Arg251, and Lys253 of cytochrome P450 1A2 on the catalytic activities and the bindings of bifunctional axial ligands.

A G Krainev et al.

Archives of biochemistry and biophysics, 298(1), 198-203 (1992-10-01)

Some eukaryotic cytochromes P450 (P450s) have a series of ionic amino acids, corresponding to Lys250, Arg251, and Lys253 residues in the P450 1A2 sequence. To understand the roles of those ionic amino acids in the catalytic function of P450, three

Inhibition of human and rat CYP1A2 by TCDD and dioxin-like chemicals.

D F Staskal et al.

Toxicological sciences : an official journal of the Society of Toxicology, 84(2), 225-231 (2005-01-22)

Dioxins have been shown to bind and induce rodent CYP1A2, producing a dose-dependent hepatic sequestration in vivo. The induction of CYP1A2 activity has been used as a noninvasive biomarker for human exposure to dioxins; while there is a consistent relationship

Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation.

A Toby A Jenkins et al.

Bioelectrochemistry (Amsterdam, Netherlands), 68(1), 67-71 (2005-06-07)

This paper describes the utilisation of methoxy-resorufin ether as an electrochemical probe for studying cytochrome P450 CYP6G1. Methoxy-resorufin ether is well established as a versatile substrate for cytochrome P450, as its demethylated product, resorufin, is a fluorophore. We show that

Sequencing and characterization of mixed function monooxygenase genes CYP1A1 and CYP1A2 of Mink (Mustela vison) to facilitate study of dioxin-like compounds.

Xiaowei Zhang et al.

Toxicology and applied pharmacology, 234(3), 306-313 (2008-12-02)

As part of an ongoing effort to understand aryl hydrocarbon receptor (AhR) mediated toxicity in mink, cDNAs encoding for CYP1A1 and the CYP1A2 mixed function monooxygenases were cloned and characterized. In addition, the effects of selected dibenzofurans on the expression

Potential impact of steatosis on cytochrome P450 enzymes of human hepatocytes isolated from fatty liver grafts.

M Teresa Donato et al.

Drug metabolism and disposition: the biological fate of chemicals, 34(9), 1556-1562 (2006-06-10)

Liver grafts discarded for transplantation because of macrosteatosis can constitute a valuable source of human hepatocytes for in vitro metabolic and pharmacotoxicological studies or for therapeutic applications. A condition for using hepatocyte suspensions for these purposes is the preservation of

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