55689

Alcohol Dehydrogenase equine

recombinant, expressed in E. coli, ≥0.5 U/mg

• Sinónimos: ADH
• Número de CAS: 9031-72-5
• Comisión internacional de enzimas: 1.1.1.1 (BRENDA, IUBMB)
• Número CE: 232-870-4
• Número MDL: MFCD00081305
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: equine
• recombinante: expressed in E. coli
• descripción: Isozyme E sequence
• formulario: lyophilized powder
• actividad específica: ≥0.5 U/mg
• color: white; light yellow
• pH: 7
• solubilidad: water: 5 mg/mL
• aplicaciones: life science and biopharma
• temp. de almacenamiento: −20°C
• Información sobre el gen: equine ... ADH1(111772995)

Descripción

Descripción general

Research Area: Neuroscience
Alcohol dehydrogenase is a zinc metalloprotein that forms five classes of isoenzymes through the dimerization of eight different subunits.

Aplicación

Alcohol Dehydrogenase equine has been used in in vitro alcohol dehydrogenase (Adh) assay.

Acciones bioquímicas o fisiológicas

Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. It has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes which create the binding site for the alcohol substrate.

Horse liver alcohol dehydrogenase (HL-ADH) is an enzyme with broad specificity, capable of catalyzing the reversible oxidation of a wide variety of primary and secondary alcohols to form their corresponding aldehydes and ketones. Moreover, alcohol dehydrogenase can oxidize ethanol while simultaneously reducing nicotinamide adenine dinucleotide (NAD+) to NADH. Previous studies have demonstrated that ADH and ALDH variants can influence alcohol dependence. Additionally, the ADH genotype has been linked to lacunar infarction and neuropsychiatric diseases.

Definición de unidad

1 U corresponds to the amount of enzyme which reduces 1 μmol benzaldehyde per minute at pH 7.0 and 30 °C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable