The recommended procedure for a 5-minute incubation in 1X Citrate Phosphate Buffer solution should be followed, with adjustments made based on the degree of staining observed with the slides. The slides should be agitated at 1 and 3 minutes, and the user should determine the amount of agitation required based on their observations. The degree of agitation may need to be adjusted to address issues such as hard-to-observe ghost cells or an excess of adult hemoglobin.
2851
Citrate Phosphate Buffer Concentrate
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 105,00 € |
Acerca de este artículo
NACRES:
NA.47
UNSPSC Code:
41116124
105,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
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solution
Quality Segment
IVD
for in vitro diagnostic use
application(s)
hematology
histology
shipped in
wet ice
storage temp.
2-8°C
Application
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Preparation Note
Sodium citrate, 0.7 mol/L, and sodium phosphate, 0.6 mol/L
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Este artículo | |||
|---|---|---|---|
| Quality Level 500 | Quality Level 500 | Quality Level 100 | Quality Level 500 |
| form solution | form powder | form liquid | form solution |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. room temp |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD - | IVD for in vitro diagnostic use |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
10 - Combustible liquids
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 112062-5G | 04061837005244 |
| 2851-100ML | 04061826259542 |
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Is it advisable to switch to the published procedure, which requires a 5-minute incubation, considering the change in cell appearance after immersing the slides for 2 minutes in the buffer?
1 respuesta-
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Are adult red blood cells failing to properly elute hemoglobin after incubation in the Citrate Phosphate Buffer working solution? Could the pH of the Citrate Phosphate Buffer be too high? Additionally, is it feasible to increase the amount of agitation to aid in eluting the adult hemoglobin?
1 respuesta-
To elute adult hemoglobin, pure/undenatured 80% alcohol is necessary. The use of histological or reagent grade alcohol may result in inadequate elution.
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