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Merck

S5133

Sigma-Aldrich

Schiff′ fuchsin-sulfite reagent

liquid

Sinónimos:

Schiff reagent

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About This Item

Fórmula empírica (notación de Hill):
C20H21N3O3S
Peso molecular:
383.46
Código UNSPSC:
12171500
NACRES:
NA.47

product name

Schiff′ fuchsin-sulfite reagent, suitable for detection of glycoproteins

Análisis

3-5 mM iodine (I) basis (titration)

Nivel de calidad

formulario

liquid

color

colorless

solubilidad

water: soluble

densidad

0.990 g/cm3

idoneidad

suitable for detection of glycoproteins

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

2-8°C

Descripción general

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Aplicación

Schiff′s fuchsin-sulfite reagent has been used:

  • in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins
  • to stain ovaries and testis
  • to stain tomato and Medicago primary roots
  • to stain undifferentiated sarcomas (USARCs)
It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Nota de preparación

Prepared using pararosaniline.

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Carc. 1B - Met. Corr. 1

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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