PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• Sinónimos: PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• Código UNSPSC: 12161700
• NACRES: NA.21

Propiedades

• Nombre del producto: Disolución salina tamponada con fostato al 0,05 % TWEEN^® 20, pH 7,4, pHast Pack^™, powder
• Línea del producto: pHast Pack^™
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry)
• impurezas: ≤5 ppm Heavy Metals (lead)
• color: white
• pH: 7.2—7.6 (25 °C)
• solubilidad: soluble, clear to hazy, colorless
• trazas de catión: Fe: <10 ppm
• actividad extraña: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temp. de almacenamiento: room temp

Descripción

Descripción general

Phosphate-buffered saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Aplicación

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2[1].

Características y beneficios

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Envase

Foil pouches

Reconstitución

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Información legal

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable