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E1903

Sigma-Aldrich

Ethidium homodimer I solution

≥95% purity (HPLC), liquid

Sinónimos:

EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride

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About This Item

Fórmula empírica (notación de Hill):
C46H50Cl4N8
Peso molecular:
856.75
Número CE:
200-664-3
Número MDL:
MFCD00210948
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24894444
NACRES:
NA.47

product name

Ethidium homodimer I solution, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO

Nivel de calidad

100

Análisis

≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)

formulario

liquid

concentración

2 mM in DMSO

color

red

ε (coeficiente de extinción)

≥9000 at 490-496 nm in water

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

−20°C

cadena SMILES

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

Clave InChI

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Categorías relacionadas

Aplicación

Ethidium homodimer I solution has been used to check membrane integrity in sperms.
Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.
Used to detect DNA in solution.

Acciones bioquímicas o fisiológicas

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.
One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

185.0 °F - closed cup

Punto de inflamabilidad (°C)

85 °C - closed cup

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
MKCV8458
MKCT2329
MKCR9828
MKCR3087
MKCS7080

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Sitaula R, et al.
Cryobiology, 58, 322-330 (2009)
C Carlsson et al.
Nucleic acids research, 23(13), 2413-2420 (1995-07-11)
Many bis-intercalating dyes used for fluorescence detection of DNA in electrophoresis have been reported to give band-splitting and band-broadening, which results in poor resolution and a decreased detection sensitivity. We have studied the dimeric dye YOYO-1, and to some extent
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Bioorganic & medicinal chemistry, 3(6), 701-711 (1995-06-01)
The binding of phenanthridine dyes to triplex poly(dT)*poly(dA).poly(dT) and its precursor duplex poly(dA).poly(dT) is characterized using linear dichroism and circular dichroism spectroscopy, and thermal denaturation. The two monomeric dyes ethidium bromide and propidium iodide are shown to behave similarly to
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PloS one, 14(10), e0215114-e0215114 (2019-10-11)
Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis; however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries or low-resource settings. Although significant advances have decreased their size, cost and accessibility

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