76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Sinónimos: Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin
• Código UNSPSC: 41171607
• NACRES: NA.85

Propiedades

• Agency: according to ISO 16654:2001
• Nivel de calidad: 200
• esterilidad: non-sterile
• formulario: powder
• caducidad: limited, expiry date on the label
• composición: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin, 0.02 g/L ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• fabricante / nombre comercial: NutriSelect^® Plus
• envase: pkg of -500 g
• condiciones de almacenamiento: (Dry place, tightly sealed containers)
• técnicas: microbiological culture: suitable
• color: faint brown
• pH final: 7.3±0.2 (25 °C)
• aplicaciones: agriculture; food and beverages; microbiology; pathogen testing
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• idoneidad: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Descripción

Descripción general

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Aplicación

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Nota de preparación

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Nota al pie de página

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Información legal

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)