18248

Protein Disulfide Isomerase from bovine liver

≥150 U/mg protein (protein ca. 40%)

• Sinónimos: PDI
• Número de CAS: 37318-49-3
• Comisión internacional de enzimas: 5.3.4.1 (BRENDA, IUBMB)
• Número MDL: MFCD00163364
• Código UNSPSC: 12352204

Propiedades

• formulario: powder; powder with small lumps
• actividad específica: ≥150 U/mg protein (protein ca. 40%)
• mol peso: 107 kDa by gel filtration; 57 kDa by SDS-PAGE
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• Clave InChI: DHUYKLYJBKXDBM-UHFFFAOYSA-N

Descripción

Propiedades físicas

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Definición de unidad

1 unit corresponds to the amount of enzyme, which, in a glutathione-dithiothreitol-system, causes a change of absorbance of 0.001 per minute at pH 4.0 and 25°C.

Otras notas

Enzyme that accelerates the exchange reaction between disulfide bridges in proteins