ERT-RO
Roche
Expand™ Reverse Transcriptase
solution, recombinant, expressed in E. coli (AP401 (k)), sufficient for 20 reactions, sufficient for 100 reactions
Sinónimos:
reverse transcriptase, expand
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
recombinante
expressed in E. coli (AP401 (k))
formulario
solution
uso
sufficient for 100 reactions
sufficient for 20 reactions
envase
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
fabricante / nombre comercial
Roche
Parámetros
42-43 °C optimum reaction temp.
técnicas
RT-PCR: suitable
RT-qPCR: suitable
entrada
purified RNA
método de detección
probe-based
temp. de almacenamiento
−20°C
Descripción general
Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
Especificidad
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Aplicación
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
Características y beneficios
- Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
- No RNase H activity
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Ideal for two-Step RT-PCR assay
- Purification of cDNA before PCR reaction is not necessary
Envase
1 set containing 3 components
Definición de unidad
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.
Volume Activity: 50 U/μl
Volume Activity: 50 U/μl
Otras notas
For life science research only. Not for use in diagnostic procedures.
Información legal
Expand is a trademark of Roche
Solo componentes del kit
Referencia del producto
Descripción
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°C)
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Edwin Yunhao Gong et al.
Methods in molecular biology (Clifton, N.J.), 1030, 195-203 (2013-07-04)
We have developed a duplex real-time RT-PCR assay for profiling antiviral inhibitors of four dengue virus (DENV) serotypes. In this assay, the primers and the probe for amplifying DENV were designed in the conserved regions of the genome after aligned
M Hatzfeld et al.
The Journal of cell biology, 149(1), 209-222 (2000-04-04)
Plakophilin 1, a member of the armadillo multigene family, is a protein with dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo.
Kathrin Ikels et al.
PloS one, 9(8), e105349-e105349 (2014-08-22)
The metabolic syndrome is a worldwide problem mainly caused by obesity. FTO was found to be a obesity-risk gene in humans and FTO deficiency in mice led to reduction in adipose tissue. Thus, FTO is an important factor for the
André Spychala et al.
PloS one, 14(2), e0211937-e0211937 (2019-02-08)
Initially, the function of the fat mass and obesity associated (Fto) gene seemed to be primarily the regulation of the body weight. Here we show that loss of Fto results in a hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis. In consequence
Christoph Gerhardt et al.
PloS one, 8(2), e57545-e57545 (2013-03-08)
Ventricular septal defects (VSDs) are the most common congenital heart defects in humans. Despite several studies of the molecular mechanisms involved in ventricular septum (VS) development, very little is known about VS-forming signaling. We observed perimembranous and muscular VSDs in
Contenido relacionado
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico