DTTP-RO

dTTP

PCR Grade, sodium salt

• Sinónimos: Thymidine 5′-triphosphate sodium salt solution, TTP
• Número de CAS: 18423-43-3
• Número MDL: MFCD00080338
• Código UNSPSC: 12352302
• ID de la sustancia en PubChem: 329798929

Propiedades

• Nivel de calidad: 100
• Análisis: 99% (dNTP)
• formulario: solution
• uso: sufficient for 125,000 standard reactions (03732711001); sufficient for 31,250 standard reactions (11969048001); sufficient for 6,250 standard reactions (11934546001)
• envase: pkg of 1,250 μL (11969048001 [100 mM]); pkg of 4 × 1,250 μL (03732711001 [4x100 mM]); pkg of 250 μL (11934546001 [100 mM])
• fabricante / nombre comercial: Roche
• concentración: <0.9% (dNDP); 100 mM
• temp. de almacenamiento: −20°C
• cadena SMILES: [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• Clave InChI: KAELIXTVSANDSE-UHFFFAOYSA-N

Descripción

Descripción general

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Aplicación

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Calidad

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Forma física

solution of dTTP in water (pH 8.3).

Nota de preparación

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Otras notas

For general laboratory use.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash