11758241001
Roche
β-Gal Reporter Gene Assay, chemiluminescent
sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)
Sinónimos:
galactosidase
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About This Item
Código UNSPSC:
23201100
Productos recomendados
uso
sufficient for 250 assays (tubes)
sufficient for 500 assays (microplate)
fabricante / nombre comercial
Roche
temp. de almacenamiento
2-8°C
Descripción general
The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.
Aplicación
The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Características y beneficios
- Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
- High dynamic measuring range: Linear range over four to five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
- Safe: No radioactive isotopes are used
- Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
- Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control
Envase
1 kit containing 7 components.
Nota de preparación
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Solo componentes del kit
Referencia del producto
Descripción
- β-Gal Substrate, Galacton Plus 100x concentrated
- Assay Buffer
- Enhancer
- Initiation Solution
- Protease Inhibitors
- Lysis Buffer 5x concentrated
- Positive Control, β-galactosidase (E. coli), lyophilizate
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Clasificaciones de peligro
Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Órganos de actuación
Central nervous system
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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