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Merck

11426320001

Roche

Anti-Fluorescein

from mouse IgG1 (clone B13-DE1)

Sinónimos:

antibody

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About This Item

Código UNSPSC:
12352200

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

B13-DE1, monoclonal

formulario

lyophilized

envase

pkg of 100 μg

fabricante / nombre comercial

Roche

isotipo

IgG1

temp. de almacenamiento

2-8°C

Descripción general

Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.

Contents
Lyophilizate, stabilized

Especificidad

The monoclonal antibody reacts with free and bound fluorescein.

Aplicación

Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
Anti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens.

Nota de preparación

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.

Reconstitución

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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