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SCM302

Sigma-Aldrich

Definitive Endoderm Induction Medium

Sinónimos:

Definitive Endoderm Medium, Endoderm Induction Medium, Medium for Endoderm Induction

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.71

formulario

liquid

envase

pkg of 50 mL

técnicas

cell culture | stem cell: suitable

impurezas

<2 EU/mL endotoxin

pH

7.0-7.4

aplicaciones

cell analysis

Categorías relacionadas

Descripción general

The definitive endoderm generates the epithelial lining of the respiratory and digestive tracts as well as cell of the thyroid, thymus, lungs, liver and pancreas. The definitive endoderm induction medium (SCM302) is a defined, animal component-free, ready-to-use serum-free medium for efficient differentiation of human pluripotent stem cells (ES/iPSCs) to definitive endoderm (DE) cells.

The 3-day differentiation protocol is simple and has been validated on multiple human iPS cell lines. Differentiated cells express high levels (>85%) of endoderm markers including CXCR4, c-Kit, Sox-17 and FOXA2 and can be further differentiated into specialized endodermal cell lineages. Definitive Endoderm Induction Medium can be used to generate 3D organoids such as hiPS derived colon (Cat. No. SCC300) and lung organoids.

Aplicación

Ready-to-use serum-free media for the efficient differentiation of human pluripotent stem cells (ES/iPSCs) to CXCR4+/c-Kit+/Sox-17+/FOXA2+ definitive endoderm (DE) cells.

Research Category:
Stem Cell Research

Calidad

Appearance: Clear liquid/no particulates
Sterility Tested: No growth
Endotoxin: <2 EU/mL
pH: 7.0-7.4
Mycoplasma: Negative
Functional Assay: >85% CXCR4+ c-Kit+ double positive after 3 days of induction.

Almacenamiento y estabilidad

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Protocolos

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

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