SCC157

6CFSMEo- Human Cystic Fibrosis Submucosal Gland Epithelial Cell Line

Human

• Sinónimos: 6CFSMEO
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: human

Propiedades

• Nombre del producto: 6CFSMEo- Human Cystic Fibrosis Submucosal Gland Epithelial Cell Line, 6CFSMEo- human CF submucosal gland epithelial cell line was derived from a cystic fibrosis patient who was compound heterozygote for the ΔF508 and Q2X CFTR mutations.
• biological source: human
• technique(s): cell based assay: suitable, cell culture | mammalian: suitable
• shipped in: ambient
• storage temp.: -140 to -196°C

Descripción

General description

Cystic fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. In CF, altered Cl- transport and secretion results in the production of thick and viscous mucus that can damage many of the body’s organs. Tracheobronchial submucosal glands secrete mucins and antimicrobial substances that keep the airways sterile along with fluids that help hydrate airway surfaces. The relationship between CF and mucus secretion is unclear and require further investigations .

6CFSMEo- is a human tracheobronchial submucosal gland epithelial cell line isolated from an individual with CF, who was compound heterozygote for the ΔF508 and Q2X CFTR mutations . ΔF508 mutation is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (ΔF508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles . Q2X mutation is a rare CF mutation in exon 1 of the CFTR gene in which the second codon (CAG) is mutated into the stop codon UAG. The 6CFSMEo- cell line was immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) .

6CFSMEo- retains the characteristic cobblestone morphology of epithelial cells along with cytokeratin expression and the ability to form tight junctions. The cell line expresses vestigial amounts of CFTR mRNA transcripts but does not express detectable levels of CFTR protein . 6CFSMEo- lacks cAMP-induced Cl- currents .

Application

6CFSMEo- human CF submucosal gland epithelial cell line was derived from a cystic fibrosis patient who was compound heterozygote for the ΔF508 and Q2X CFTR mutations.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Biochem/physiol Actions

Epithelial Cells

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable