MABT853M

Anti-Podoplanin Antibody, clone LpMab-19

clone LpMab-19, from mouse

• Sinónimos: Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: LpMab-19, monoclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin)
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_006465.3
• Nº de acceso UniProt: Q86YL7
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PDPN(10630)

Descripción

Descripción general

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. A high degree of expression is observed in placenta, lung, skeletal muscle, and brain. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-19 recognizes Sialylated O-Glycan on Thr76 of human Podoplanin. Its reactivity with Podoplanin is lost following T76A mutation. LpMab-19 recognizes endogenous hPDPN that is expressed in LN319 glioblastoma cell line, but fails to react with LN319 cells with hPDPN knock out. The minimum epitope of LpMab-19 is identified to be Thr76-Arg79 hPDPN. (Ref.: Ogasawara, S., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(5); 245-253).

Especificidad

Clone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.

Inmunógeno

LN229 glioma cells expressiing human Podoplanin.

Aplicación

Anti-Podoplanin, clone LpMab-19, Cat. No. MABT853, is a mouse monoclonal antibody that detects human Podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue.

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(5); 245-253.

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(5); 245-253.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother. 35(5); 245-253.

Research Category
Cell Structure

Calidad

Evaluated by Immunohistochemistry in human testis cancer tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human testis cancer tissue.

Descripción de destino

16.70 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1