MABS1272

Anti-Endothelial Protein C Receptor Antibody, clone 1560

clone 1560, from rat

• Sinónimos: Activated protein C receptor, APC receptor, Centrocyclin, Centrosomal protein CCD41, Endothelial cell protein C receptor, CD201
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1560, monoclonal
• reactividad de especies: mouse
• técnicas: flow cytometry: suitable; inhibition assay: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_035301
• Nº de acceso UniProt: Q64695
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Procr(19124)

Descripción

Descripción general

Endothelial protein C receptor (UniProt: Q64695; also known as EPCR, Activated protein C receptor, APC receptor, Centrocyclin, Centrosomal protein CCD41, Endothelial cell protein C receptor, CD201) is encoded by the Procr (also known as Epcr) gene (Gene ID: 19124) in murine species. EPCR is a multi-functional single-pass type I membrane protein that is expressed in endothelial cells. Its expression has also been reported in hematopoietic, neuronal, epithelial progenitor cells, and breast cancer stem cells. It binds to activated protein C and enhances protein C activation by the thrombin-thrombomodulin complex and thereby plays a role in the protein C pathway controlling blood coagulation. It is also shown to play a role in mediating activated protein C-induced cytoprotective effects, including antiapoptotic, anti-inflammatory, and barrier stabilization. EPCR exhibits sequence and 3-dimensional structural homology with the major histocompatibility class 1/CD1 family of proteins, particularly CD1d. It contains a tightly bound phospholipid in the antigen presenting groove and phosphatidylcholine is shown to be the major phospholipid bound to human EPCR. Removal of this lipid results in the loss of protein C binding.

Especificidad

Clone 1560 specifically detects endothelial protein C receptor in murine species.

Inmunógeno

A full length recombinant mouse endothelial protein C receptor.

Aplicación

Anti-Endothelial Protein C Receptor, clone 1560, Cat. No. MABS1272, is a rat monoclonal antibody for detection of murine Endothelial Protein C Receptor and is tested for use in Flow Cytometry and Inhibition Assays.

Flow Cytometry Analysis: A representative lot detected Endothelial Protein C Receptor in Flow Cytometry applications (Li, W., et. al. (2005). J Thromb Haemost. 3(7):1351-9; Balazs, A.B., et. al. (2006). Blood. 107(6):2317-21).

Inhibition Analysis: A representative lot detected Endothelial Protein C Receptor in Inhibition applications (Li, W., et. al. (2005). J Thromb Haemost. 3(7):1351-9; Disse, J., et. al. (2011). J Biol Chem. 286(7):5756-67).

Research Category
Signaling

Calidad

Evaluated by Flow Cytometry in bEND.3 mouse endothelial cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Endothelial Protein C Receptor in one million bEND.3 mouse endothelial cells.

Descripción de destino

27.19 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in PBS without preservatives.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable