MABS1235

Anti-Progesterone Receptor A/B Antibody, clone 488/H3

clone 488/H3, from mouse

• Sinónimos: Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, Progesterone Receptor A/B
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 488/H3, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_000917; NP_001189403
• Nº de acceso UniProt: P06401
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PGR(5241)

Descripción

Descripción general

Progesterone receptor (UniProt P06401; also known as nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B, but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.

Especificidad

Clone 488/H3 reacts with both isoform 1 (isoform B; PR-B) and isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).

Inmunógeno

Recombinant protein corresponding to the N-terminal half fragment of human Progesterone Receptor B.

Aplicación

Immunocytochemistry Analysis: A representative lot detected exogenously expressed progesterone receptor isoform A and isoform B (PR-A & PR-B) in transfected MCF10A cells by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoforms A & B (PR-A & PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-A & PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).

This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.

Calidad

Evaluated by Western Blotting in human breast cancer T47D cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected both isoforms A and B (PR-A & PR-B) of progesterone receptor in human breast cancer T47D cell lysate.

Descripción de destino

~110/80 kDa observed

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable