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MABN40

Sigma-Aldrich

Anti-LRRK2 Antibody, clone N138/6

clone N138/6, from mouse

Sinónimos:

leucine-rich repeat kinase 2, DARDARIN, augmented in rheumatoid arthritis 17, Parkinson disease (autosomal dominant) 8, Dardarin

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

100

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

N138/6, monoclonal

reactividad de especies

human

reactividad de especies (predicha por homología)

mouse (based on 100% sequence homology)

técnicas

immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

NP_940980

Nº de acceso UniProt

Q5S007

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... LRRK2(120892)

Descripción general

Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.

Especificidad

This antibody recognizes LRRK2.

Inmunógeno

Recombinant protein corresponding to human LRRK2.

Aplicación

Anti-LRRK2 Antibody, clone N138/6 detects level of LRRK2 & has been published & validated for use in IH & WB.
Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, neuromab@ucdavis.edu)

Calidad

Evaluated by Immunohistochemistry in human placenta and Parkinson′s diseased vascular brain tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected LRRK2 on 10 µg of human placenta and Parkinson′s diseased vascular brain tissue.

Descripción de destino

240 kDa observed. 286 kDa calculated. (Glasson, B., et al. (2006). Ann. Neurol. 59(2):315-322.)

Forma física

Format: Purified

Nota de análisis

Control
Human placenta and Parkinson′s diseased vascular brain tissue

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Iakov N Rudenko et al.
The Biochemical journal, 474(9), 1547-1558 (2017-03-23)
Autosomal dominant mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson's disease (PD). Most pathogenic LRRK2 mutations result in amino acid substitutions in the central ROC (Ras of complex proteins)-C-terminus of ROC-kinase triple domain and affect enzymatic functions
Ruth Chia et al.
Nature communications, 5, 5827-5827 (2014-12-17)
LRRK2, a gene relevant to Parkinson's disease, encodes a scaffolding protein with both GTPase and kinase activities. LRRK2 protein is itself phosphorylated and therefore is subject to regulation by cell signalling; however, the kinase(s) responsible for this event have not

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