MABN2436

Anti-Guanylate Cyclase 2E Antibody, clone 8A5

clone 8A5, from mouse

• Sinónimos: RETGC-1, Guanylate cyclase-E, Guanylate cyclase-2E, GC-E
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 8A5, monoclonal
• reactividad de especies: bovine, mouse, human
• envase: antibody small pack of 25 μg
• técnicas: immunofluorescence: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_032218
• Nº de acceso UniProt: P52785
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Gucy2E(14919)

Descripción

Descripción general

Guanylyl cyclase GC-E (UniProt: P52785; also known as EC: 4.6.1.2, Guanylate cyclase 2E, GC-2E) is encoded by the Gucy2e (also known as Guc2e) gene (Gene ID: 14919) in murine species. GC-2E is a single-pass type I membrane protein that plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the re-synthesis of cGMP required for recovery of the dark state after phototransduction. Following photoexcitation, cGMP in photoreceptors is first hydrolyzed by a light-activated phosphodiesterase (PDE6) and then replenished by a retinal membrane guanylyl cyclase. It is synthesized with a 50 amino acid signal peptide that is cleaved to generate the mature enzyme. It consists of an extracellular domain (aa 51-469), a helical domain (aa 470-490) and a cytoplasmic domain (aa 491-1108).

Especificidad

Clone 8A5 detects Guanylate cyclase-1 in Bovine, Human, and murine species. It targets an epitope within 48 amino acids from the C-terminal end.

Inmunógeno

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 48 amino acids from the C-terminal end of human Guanylate cyclase-1.

Aplicación

Anti-Guanylate Cyclase-2E, clone 8A5, Cat. No. MABN2436, is a highly specific mouse monoclonal antibody that targets retinal Guanylyl cyclase (RETGC-1; GC-2E) and has been used in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in mouse retina tissues (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in Immunofluorescence applications (Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905).

Western Blotting Analysis: 10 ug/mL of a representative lot detected Guanylate Cyclase-1 in mouse retina tissue lysates (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Western Blotting Analysis: A representative lot detected Guanylate Cyclase-1 in Western Blotting applications (Peshenko, I.V., et. al. (2011). Biochemistry. 50(44):9511-9; Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905)

Research Category
Neuroscience

Calidad

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Descripción de destino

~120 kDa observed; 120.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1