MABN1846

Anti-Apelin Receptor/APJ, clone 3C3-7 Antibody

clone 3C3-7, from mouse

• Sinónimos: Apelin receptor, Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3C3-7, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_005152
• Nº de acceso UniProt: P35414
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... APLNR(187)

Descripción

Descripción general

Apelin receptor (UniProt P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID 187) in human. Apelin is an angiotensin converting enzyme 2 (ACE2) substrate that functions as an inotropic and cardiovascular protective peptide. Apelin exerts its physiological effects via its receptor APJ, a seven-transmembrane G protein-coupled receptor that shares significant homology with the Ang II type 1 receptor (AT1R). Apelin/APJ signaling plays an important role in a wide range of pathophysiological effects in the cardiovascular system. Apelin levels are reduced in patients with essential hypertension independent of left ventricular systolic and diastolic dysfunction. Treatment with Apelin has been shown to lower systolic blood pressure levels in hypertensive rats via an NO-dependent mechanism. APJ is a 7-transmembrane (a.a. 27-51, 67-91, 101-125, 145-166, 201-221, 245-271, 285-308) GPCR, having a short N-terminal extracellular portion (a.a. 1-26) and a long C-terminal cytomplasmic tail (a.a. 309-380).

Especificidad

Clone 3C3-7 detects human Apelin receptor/APJ.

Inmunógeno

Recombinant full-length human Apelin receptor/APJ.

Aplicación

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Apelin receptor/APJ in human prostate cancer tissue, as well as normal cerebral cortex and pancreas tissue sections.

Research Category
Neuroscience

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Apelin Receptor, clone 3C3-7 Antibody is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of APJ.

Calidad

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Apelin receptor/APJ in 10 µg of MCF7 cell lysate.

Descripción de destino

~42 kDa 42.66 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable