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MABF431

Sigma-Aldrich

Anti-CD45.1 Antibody (mouse), PerCP-Cy5.5, clone A20

clone A20, 0.2 mg/mL, from mouse

Sinónimos:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.44

origen biológico

mouse

Nivel de calidad

conjugado

PerCP-Cy5.5

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

A20, monoclonal

reactividad de especies

mouse

concentración

0.2 mg/mL

técnicas

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotipo

IgG2aκ

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

mouse ... Ptprc(19264)

Descripción general

The A20 antibody reacts with mouse CD45.1, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Inmunógeno

Immunogen information for this antibody is proprietary information.

Aplicación

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD45.1 (mouse), PerCP-Cy5.5, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.

Calidad

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.1 in one million C57Bl/6 or SJL splenocytes.

Forma física

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Almacenamiento y estabilidad

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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