MABF3137

Anti-MXRA8 Antibody, clone 9E9

• Sinónimos: Dual Ig domain-containing cell adhesion molecule, Limitrin, Matrix remodeling-associated protein 8
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 9E9, monoclonal
• mol peso: calculated mol wt 49.13 kDa
• purificado por: using protein G
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse
• envase: antibody small pack of 100
• técnicas: flow cytometry: suitable; inhibition assay: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NP_001269511
• Nº de acceso UniProt: Q9BRK3
• temp. de almacenamiento: -10 to -25°C
• Información sobre el gen: human ... MXRA8(54587)

Descripción

Especificidad

Clone 9E9 is a mouse monoclonal antibody that detects Matrix remodeling-associated protein 8 (MXRA8). It targets an epitope within 15 amino acids from the N-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain within the N-terminal region of human Matrix remodeling-associated protein 8 (MXRA8).

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected MXRA8 in one million THP-1 cells.

Tested Applications

Inhibition Assay: A representative lot block MXRA8 activity and reduced TH17 cell trafficking across the blood brain barrier. (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

Flow Cytometry Analysis: A representative lot detected MXRA8 in Flow Cytometry applications (Charabati, M., et al. (2022). Sci Transl Med.14(626): eabj0473).

Western Blotting Analysis: A representative lot detected MXRA8 in Western Blotting applications (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Matrix remodeling-associated protein 8 (UniProt: Q9BRK3; also known as Dual Ig domain-containing cell adhesion molecule, DICAM, MXRA8, Limitrin) is encoded by the MXRA8 gene (Gene ID: 54587) in human. MXRA8/DICAM is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-341), a transmembrane domain (aa 342-362), and a cytoplasmic domain (aa 363-442). It is highly expressed at the areas of cell-cell contacts. It is also detected in the soluble fraction of the nucleus. Its high expression is observed in the brain where it localizes to glia limitans formed by the end feet of astrocytes surrounding capillaries, and beneath the pia mater. It is also expressed in intercalated disks in the heart and has been detected in pancreatic -cells. It modulates the activity of various signaling pathways via binding to integrin V:β3 via its RGD motif (aa 252-254). It plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes and stimulates the formation of primary cilia in chondrocytes. MXRA8 also plays a role in the maturation and maintenance of the blood-brain barrier. It is reported to function as a cell trafficking molecule that is preferentially expressed by T helper 17 (TH17)-polarized CD4+ T lymphocytes. Its expression on circulating CD4+ T cells is increased in patients with active relapsing-remitting multiple sclerosis (RRMS) and in progressive forms of multiple sclerosis. Expression of its ligands also increases on the blood-brain barrier endothelium upon inflammation and in multiple sclerosis lesions. Four isoforms of MXRA8 have been described that are produced by alternative splicing. (Ref.: Charabati, M., et al. (2022). Sci. Transl. Med. 14; eabj0473; Zhang, R., et al. (2018). Nature. 557(7706); 570-574).

Forma física

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable