MABF28

Anti-PAR-3 Antibody, clone 8E8

clone 8E8, from mouse

• Sinónimos: Proteinase-activated receptor 3, Coagulation factor II receptor-like 2, Thrombin receptor-like 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 8E8, monoclonal
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human (based on 100% sequence homology)
• técnicas: activity assay: suitable; flow cytometry: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_004092
• Nº de acceso UniProt: O00254
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PARD3(56288)

Descripción

Descripción general

PAR-3 (also known as Coagulation factor II receptor-like 2, Thrombin receptor-like 2 or F2RL2) is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR-3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR-3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR-3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR-3 interacts with PRCKI. PAR-3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR-3 interacts with F11R/JAM1. Antibodies against PAR-3 are present in sera from patients with cutaneous T cell lymphomas.

Inmunógeno

KLH-conjugated linear peptide corresponding to human PAR-3.

Aplicación

Anti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)

Activity Assay Analysis (platelet aggregation): A previous lot was used by an independent laboratory in platelet aggregation assay. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)

Research Category
Infectious Diseases

Research Sub Category
Inflammation & Autoimmune Mechanisms

Calidad

Evaluated by Flow Cytometry in mouse platelets from washed whole blood (heparinized).

Flow Cytometry Analysis: 2 µg of this antibody detected PAR-3 in mouse platelets from washed whole blood (heparinized).

Descripción de destino

40 kDa calculated

Ligadura / enlace

Replaces: MABS174

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Mouse platelets from washed whole blood (heparinized)

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable