MABF2761

Anti-CXCL1/GROa Antibody, clone HL2401

• Sinónimos: C-X-C motif chemokine 1;GRO-alpha(1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: HL2401, monoclonal
• mol peso: calculated mol wt 11.3 kDa; observed mol wt ~15 kDa
• purificado por: affinity chromatography
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: ELISA: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_001502
• Nº de acceso UniProt: P09341
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CXCL1(2919)

Descripción

Descripción general

Growth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, CXCL1, GRO-alpha(1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1 is a multi-functional chemokine that displays chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein that can be further cleaved into three chains (aa 4-73; 5-73; and 6-73) via proteolytic cleavage after secretion from peripheral blood monocytes. CXCL1 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Its levels are reported to be low under basal conditions but increase significantly following active infection. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1 signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1 has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. Clone HL2401 is shown to specifically binds to CXCL1, although it may show slight binding to CXCL2. It is shown to reduce both cellular proliferation and invasion of cancer cells overexpressing CXCL1. It also induces apoptosis and impairs tumor vasculature. (Ref.: Miyake, M., et al. (2019). Theranostics.;9(3):853-867; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696Wuyts, A., et al. (1999). Eur. J. Biochem. 260(2); 421-429).

Especificidad

Clone HL2401 is a mouse monoclonal antibody that detects CXCL1.

Inmunógeno

Native human CXCL1 protein.

Aplicación

Quality Control Testing

Evaluated by Western Blotting with recombinant Human CXCL1 protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL1 protein.

Tested Applications

ELISA Analysis: A representative lot detected CXCL1 in ELISA application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Inhibition Assay: A representative lot inhibited proliferation and invasion of bladder and prostate cells and disrupted endothelial sprouting in vitro. (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CXCL1 in Immunoprecipitation application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Western Blotting Analysis: A representative lot detected CXCL1 in Western Blotting application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forma física

Purified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable