MABE60

Anti-BRG1 Antibody, clone 3G4

clone 3G4, from rat

• Sinónimos: Transcription activator BRG1, ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3G4, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001122316
• Nº de acceso UniProt: P51532
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SMARCA4(6597)

Descripción

Descripción general

Transcription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.

Inmunógeno

GST-tagged recombinant protein corresponding to human BRG1.

Aplicación

Anti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Transcription Factors

Western Blotting Analysis: A representative lot detected BRG1 (SMARCA4) in THP-1 human leukemia monocytic cell lysates (Cruickshank, V.A., et al. (2015). PLoS One. 10(11):e0142806).

Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).

Calidad

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.

Descripción de destino

~185 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable