MABE2022

Anti-GINS2/PSF2 Antibody, clone 78C

• Sinónimos: DNA replication complex GINS protein PSF2;GINS complex subunit 2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 78C, monoclonal
• mol peso: calculated mol wt 21.43 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2a
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_057179
• Nº de acceso UniProt: Q9Y248
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GINS2(51659)

Descripción

Descripción general

DNA replication complex GINS protein PSF2 (UniProt: Q9Y248; also known as GINS complex subunit 2) is encoded by the GINS2 (also known as PSF2, CGI-122, DC5, HSPC037) gene (Gene ID: 51659) in human. GINS/PSF2 is a component of the GINS complex, which is a heterotetramer of GINS 1, 2, 3, and 4 that are assembled with 1:1:1:1 stoichiometry. Each of these four subunits is essential for proper completion of DNA replication initiation and elongation. This complex plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex binds preferentially to single-stranded DNA. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein Cdc45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. GINS2 gene is reported to be frequently up-regulated in the cancer cells. Transfection of ETK-1 and HuH28 cells with PSF2 siRNA is shown to reduce the amount of transcript and suppress cell growth, indicating the important role of GINS2/PF2 in development of cholangiocarcinoma. (Ref.: Aparicio, T., et al. (2009). Nucl. Acids Res. 37(7); 2087-2095; Boskovic, J., et al. (2007). EMBO Rep. 8(7); 678-684; Obama, K., et al. (2005). Oncol. Rep. 14(3):701-706).

Especificidad

Clone 78C is a mouse monoclonal antibody that detects human DNA replication complex GINS protein PSF2 (GINS2/PSF2).

Inmunógeno

Recombinant human GINS2/PSF2 expressed in E. coli.

Aplicación

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable