MABE1878

Anti-CNOT6 Antibody, clone Y6206

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies, primary antibodies
• clon: Y6206, monoclonal
• mol peso: calculated mol wt 63.3 kDa; observed mol wt ~62 kDa
• purificado por: using protein G
• reactividad de especies: mouse, human
• envase: antibody small pack of 100 μg
• técnicas: western blot: suitable
• isotipo: IgG2bκ
• secuencia del epítopo: N-terminal half
• Nº de acceso Protein ID: NP_001290170
• Nº de acceso UniProt: Q9ULM6
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... Cnot6(57472)

Descripción

Descripción general

CCR4-NOT transcription complex subunit 6 (UniProt: Q9ULM6; also known as EC:3.1.13.4, CCR4 carbon catabolite repression 4-like, Carbon catabolite repressor protein 4 homolog, Cytoplasmic deadenylase) is encoded by the Cnot6 (also known as Ccr4, Kiaa1194) gene (Gene ID: 57472) in human species. CNOT6, a catalytic component of the CCR4-NOT complex with in mammals. This complex is ubiquitously expressed and contains at least ten subunits. Subunits CNOT6, CNOT7, and CNOT8 display deadenylase activity and CNOT1, 2, 3, 9, 10, and 11 have regulatory NOT modules. CCR4-NOT complex is reported to limit expression of specific genes through deadenylation of mRNA poly(A) tails, enabling positive selection. CNOT6 is detected both in the nucleus and in cytoplasm. It also serves as a poly(A) nuclease with 3′-5′ RNase activity. It has been linked to multiple cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. The deadenylase activity of CCR4-NOT complex has also been implicated in the control of obesity and energy metabolism. In the liver, the deadenylase activity is recruited to the 3 -end of mRNAs of energy metabolism-associated genes and shortens their lifespans and reduces their expression levels. Subunits CNOT 6, 7, and 8 are also shown to participate in the regulation of induced pluripotent stem cell generation and CNOT1, 2, and 3 are involved in maintenance and self-renewal of embryonic stem cells. (Ref.: Ito-Kureha, T., et al. (2020). Nat. Commun. 11; 6169; Zukeran, A., et al. (2016). Biochem. Biophys, Res. Commun. 474; 233-239; Takahashi, A., et al. (2015). Cell Reports 13, 2756-2767).

Especificidad

Clone Y6202 is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6). It targets an epitope within the N-terminal half.

Inmunógeno

A recombinant fragment corresponding to 50 amino acids from the N-terminal half of murine CNOT6 fused with baculovirus gp64 protein.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNOT6 in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNOT6 in lysates from Mouse embryonic stem cells and DLD-1 cells.

Western Blotting Analysis: A representative lot detected CNOT6 in Western Blotting applications (Ito-Kureha, T., et al. (2020). Nat Commun.11(1):6169; Otsuka, H., et al. (2020). Biochimie.;174:49-56; Mostafa, D., et al. (2020). RNA Biol.;17(3):403-416; Zukeran, A., et al. (2016). Biochem Biophys Res Commun. 474(2):233-239; Takahashi, A., et al. (2015). Cell Rep. 13(12):2756-67).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CNOT6, clone Y6206, Cat. No. MABE1878, is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6) and is tested for use in Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable