MABE1799

Anti-Nrf2 Antibody, clone 103

clone 103, from rat

• Sinónimos: Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 103, monoclonal
• reactividad de especies: rat, mouse
• técnicas: immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_035032
• Nº de acceso UniProt: Q60795
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Nfe2L2(18024)

Descripción

Descripción general

Nuclear factor erythroid 2-related factor 2 (UniProt: Q60795; also known as Nrf2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2) is encoded by the Nfe2l2 (also known as Nrf-2, Nrf2) gene (Gene ID: 18024) in murine species. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that is involved in adaptation of cells to oxidative stress and electrophiles. It bind to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors overexpressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).

Especificidad

Clone 103 detects Nrf2 in Keap-1 knockout cells. It targets an epitope within 179 amino acids in the internal region.

Inmunógeno

GST-tagged recombinant fragment corresponding to 179 amino acids from internal region of murine Nuclear factor erythroid 2-related factor 2.

Aplicación

Anti-Nrf2, clone 103 , Cat. No. MABE1799, is a highly specific rat monoclonal antibody that targets Nuclear factor erythroid 2-related factor 2 and has been tested in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Nrf2 in Immunohistochemistry applications (Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84).

Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Western Blotting in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.

Descripción de destino

~67 kDa observed; 66.90 kDa calculated Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1