MABE1039

Anti-Nup96 Antibody, clone 4H5

clone 4H5, from rat

• Sinónimos: Nuclear pore complex protein Nup98-Nup96, Nuclear pore complex protein Nup98, 98 kDa nucleoporin, Nucleoporin Nup98, Nup98, Nuclear pore complex protein Nup96, 96 kDa nucleoporin, Nucleoporin Nup96, Nup96, Nup96
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4H5, monoclonal
• reactividad de especies: mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_001274093
• Nº de acceso UniProt: Q6PFD9
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Nup98(269966)

Descripción

Descripción general

The murine gene Nup98 (Gene ID 269966; also known as AI849286, 4732457F17) encodes the 1816-amino acid nuclear pore complex protein Nup98-Nup96 (UniProt Q6PFD9), which is posttranslationally autocleaved between aa 800 and 801 to form Nup98 (aa 1-800) and Nup96 (aa 801-1816). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. The core structure of the NPC is maintained by the Nup93-205 complex sandwiched between the supporting structures of the Nup107-160 complex on the nuclear and cytoplasmic faces of the pore. Nup96 is a component of the Nup107-160 complex, the largest subunit of the NPC. Nup96 knockout is reported to be fatal in mice and Nup96(+/-) mice with low levels of Nup96 display impaired immune response and are highly susceptible to viral infection.

Especificidad

Reacts with Nup98-Nup96 precursor (isoforms 1, 2, and 6), nucleoporin Nup96 (isoform 5), but not nucleoporin Nup98 (isoform 3).

Inmunógeno

Epitope: Near N-terminus.

GST-tagged recombinant protein corresponding to mouse Nup96 near the N-terminus.

Aplicación

Anti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
Immunocytochemistry Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).

Calidad

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nup96 in 10 µg of NIH/3T3 cell lysate.

Descripción de destino

~120 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable