MABC679

Anti-RUNX1 Antibody, clone 2B5

ascites fluid, clone 2B5, from mouse

• Sinónimos: Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2 ; ; Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 2B5, monoclonal
• reactividad de especies: human
• técnicas: western blot: suitable
• isotipo: IgG1
• Nº de acceso UniProt: Q01196
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RUNX1(861)

Descripción

Descripción general

Runt-related transcription factor 1 (RUNX1), also known as Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2 (CBF-alpha2), Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B/PEBP2-alpha B), SL3-3 enhancer factor 1 alpha B subunit, and SL3/AKV core-binding factor alpha B subunit is encoded by the human gene named RUNX1/AML1, CBFA2. RUNX1 is a transcription factor that binds to a wide variety of promoters and enhancers belonging to many types of genes from T cell receptor genes to LCK, IL-3 and GM-CSF. Its major normal role is in the development of blood cells and hematopoiesis. It is expressed in most tissues except in normal brain and heart. Levels are highest in the bone marrow, thymus and peripheral lymphocytes. Defects and or translocations involving RUNX1 are the cause for myeloid leukemias as well as lymphoblastic leukemia. Mutations in RUNX1 function are also linked with many breast cancers. Finally, mutations in RUNX1 have recently been shown to be the cause of cancers in epithelial tissues and linked to squamous cell carcinomas. EMD-Millipore’s Anti-RUNX1 mouse monoclonal antibody has been tested in western blot on Jurkat cell lysates and in ELISA using recombinant RUNX1 protein.

Inmunógeno

Synthesized peptide of human RUNX1.

Aplicación

Anti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected RUNX1 in Jurkat cell lysate.

Optimal working dilutions must be determined by end user.

Descripción de destino

~55 kDa observed. Uncharacterized bands may appear in some lysate(s).

Forma física

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Jurkat cell lysate

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable