MABC1170

Anti-MNDA Antibody, clone 3C1

clone 3C1, from rat

• Sinónimos: Myeloid cell nuclear differentiation antigen
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3C1, monoclonal
• reactividad de especies: human
• técnicas: dot blot: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_002423
• Nº de acceso UniProt: P41218
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MNDA(4332)

Descripción

Descripción general

Myeloid cell nuclear differentiation antigen (UniProt P41218) is encoded by the MNDA gene (Gene ID 4332) in human. MNDA is a member of the interferon-inducible p200 (IFI-200) family of proteins with a conserved HIN-200 domain (human MNDA a.a. 196-394) that mediate interaction with proteins and DNA. IFI-200 members possess antimicrobial, cell growth regulatory, differential regulatory and immunomodulatory properties. In bone marrow-derived- and mature neutrophils, MNDA is predominantly located in the nucleus. MNDA is cleaved by caspases in neutrophils undergoing apoptosis and relocated to the cytoplasm. MNDA is downregulated in myelodysplastic syndrome and completely absent in a mouse strain susceptible to B-cell plasmacytoma, while being markedly (1,000-fold) upregulated in a mouse strain resistant to B-cell plasmacytoma. Originally thought to be restricted to, myeloid cells, MNDA and other IFI-200 family members are also found to be expressed in a variety of non-myeloid cell types. MNDA is highly expressed in normal bone tissue, while being at markedly low levels in osteosarcoma cells. MNDA overexpression is shown to induce apoptosis, inhibit proliferation, and reduce the migration of osteosarcoma cells.

Especificidad

Clone 3C1 reacted with both denatured and non-denatured MNDA. Clone 3C1 reacted with nuclear extract from NMDA-expressing HL-60, but not non-MNDA-expressing BII, human myeloid leukemia cells (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).

Inmunógeno

Human peripheral blood granulocyte nuclei.

Aplicación

Detect MNDA using this rat monoclonal Anti-MNDA Antibody, clone 3C1, Cat. No. MABC1170, validated for use in Dot Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Dot Blot Analysis: Clone 3C1 hybridoma culture supernatant reacted with nuclear extract from HL-60, but not BII, human myeloid leukemia cells (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).

Flow Cytometry Analysis: A representative lot (pre-conjugated with Alexa Fluor^™ 488) detected MNDA expression among the granulocyte-macrophage (G-M) progenitors, but not among the nucleated red blood cells (nRBCs) and lymphoid cells in control and myelodysplastic syndromes (MDS) human marrow aspirates. A G-M progenitor cell population with downregulated MNDA expression was detected in most marrow samples from MDS cases and some MDS cases contain MNDA-negative G-M progenitor cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).

Immunocytochemistry Analysis: A representative lot detected MNDA nuclear immunoreactivity among HL-60 cells and NMDA-transfected, but not untransfected K562 cells. MNDA-negative HL-60 cells increased with time in cultures maintained without fresh medium for an extended period of time. MNDA translocated from nucleus to cytoplasm beginning at 3 hours and completed 4 hours following H2O2 addition to HL-60 cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MNDA from HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).

Western Blotting Analysis: A representative lot detected a time-dependent loss of MNDA in HL-60 cell lysate beginning at 4 hours following H2O2 treatment (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).

Western Blotting Analysis: Clone 3C1 hybridoma culture supernatant detected MNDA in HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).

Research Category
Apoptosis & Cancer

Calidad

Evaluated by Immunocytochemistry in HL-60 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected MNDA in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HL-60 cells.

Descripción de destino

45.84 kDa calculated. ~55 kDa reported (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553). Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified.

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1