MABC1023

Anti-EGR1 Antibody, clone 16A10.1

clone 16A10.1, from mouse

• Sinónimos: Early growth response protein 1, AT225, EGR-1, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 16A10.1, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001955
• Nº de acceso UniProt: P18146
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EGR1(1958)

Descripción

Descripción general

Early growth response protein 1 (UniProt P18146; also known as AT225, EGR-1, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24) is encoded by the EGR1 (also known as KROX24, ZNF225) gene (Gene ID 1958) in human. EGR-1 is the founding member of the early growth response (EGR) family of Cys2-His2-type zinc finger transcription factors that share a high homology in their DNA-binding region. EGR-1 is ubiquitously expressed in many cell types and binds to a consensus DNA motif (GCGGTGGGCG) in target genes, including c-Myc, cyclins D2 & G2, p19, TNF, IL-2, CD44, and ICAM-1. EGR1 is known as an immediate-early response gene due to the rapid kinetics of its induction by various signals, including growth factors, cytokines, and stress. Human EGR-1 is a 543-amino acid protein with three C2H2-type Zinc fingers (a.a. 338-362, 368-390, 396-418).

Inmunógeno

Epitope: Internal.

GST-tagged recombinant protein corresponding to an internal fragment of human EGR1.

Aplicación

Anti-EGR1 Antibody, clone 16A10.1 is an antibody against EGR1 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected EGF-induced upregulation of nuclear EGR1 immunoreactivity in serum-starved HeLa and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Transcription Factors

Calidad

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VEGF-induced EGR1 expression in serum-starved HUVECs.

Descripción de destino

~80 kDa observed. 57.51 kDa calculated. The observed target band size is consistent with the reported apparent molecular weight (Krones-Herzig, A., et al. (2005). Cancer Res. 65(12):5133-5143; Josefsen, K., et al. (1999). Diabetologia. 42(2):195-203).

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable