MAB5778-I

Anti-NMDAR2B Antibody, C-term Antibody, clone 1C6.5C4

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1C6.5C4, monoclonal
• mol peso: calculated mol wt 166.07 kDa; observed mol wt ~170 kDa
• reactividad de especies: rat, human, mouse, rabbit
• envase: antibody small pack of 100 μL
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso UniProt: Q00960
• Condiciones de envío: dry ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Glutamate receptor ionotropic, NMDA 2B (UniProt: Q00960; also known as GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B) is encoded by the Grin2b gene (Gene ID: 24410) in rat. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2B is a multi-pass membrane protein, which is synthesized with a signal peptide (aa 1-26), that is subsequently cleaved off to generate the mature form that contains two extracellular domains, three cytoplasmic domains, and four transmembrane domains. It is strongly expressed at birth and its levels increase until P6 to P20 and then begins to decline to the adult level. NMDAR2B is a component of the NMDA receptor complex that functions as heterotetrameric, ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. The channel activation requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. (Ref.: Laurie, DJ., et al. (1997). Mol. Brain Res. 51(1-2); 23-32).

Especificidad

Clone 1C6.5C4 is a mouse monoclonal antibody that detects NMDAR2B. It targets an epitope within 136 amino acids from the C-terminal half.

Inmunógeno

His-tagged recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat NMDAR2B.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NMDAR2B in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Laurie, D.J., et. al. (1997). Mol. Brain Res. 51(1-2):23-32).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-NMDAR2B, C-term, clone 1C6.5C4, Cat. No. MAB5778-I, is a mouse monoclonal antibody that detects NMDAR2B and is tested for use in Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable